PLFA Fingerprint Method for Assessing Microbial Communities of Strong Aromatic Liquor Pit Mud

Abstract

In the view of the specificity of microbial communities in pit mud during brewing process,different types of soil samples and common strains in liquor brewing were investigated to assess the fingerprints of phospholipid fatty acid(PLFA) of microbial communities in Luzhou flavor pit mud.The results suggested the composition and content of PLFA significantly affected by physiological and environmental conditions which resulted in the significant differences(P0.05) of PLFA fingerprints among the samples(the ratio of fungal/bacterial PLFA in garden and roadside soils were 0.30 and 0.66,respectively,which were both less than 1,the ratio in pit mud however was 1.17).The content of special PLFA changed significantly(P0.05) when five pure cultured microorganisms were added.The content of a14:0 and a16:0 in pit mud adding SICC1.13 and CICC20633 were 1.44 and 9.92 times higher than those in blank respectively,while 18:2ω6,9 in SICC3398 and SICC31482 were 82.58 and 90.33 times higher than that in the blank.Furthermore,cy17:0 was identified in the pit mud adding DH5α.Principal component analysis(PCA) was performed to evaluate the changes of special PLFA’s composition and content in pit mud after adding the microorganisms.The results of this study demonstrated that PLFA fingerprint method was a fast biochemical culture-independent method,which was beneficial to characterize the fingerprints of microbial communities during liquor process.