Pleurochrysis carterae Hot-Water Extract Inhibits Melanogenesis in Murine Melanoma Cells

Kazuomi Sato,Yuji Yamaguchi,Setsuko Sakaki,Hiroyuki Takenaka

doi:10.3390/cosmetics6040060

Kazuomi Sato, Yuji Yamaguchi + Show 2 more

Open Access

https://doi.org/10.3390/cosmetics6040060

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Journal: Cosmetics	Publication Date: Oct 18, 2019
Citations: 3	License type: CC BY 4.0

Affiliation: Akebono (Japan)

Abstract

In this study, we examined the effect of a hot-water extract of coccolithophore Pleurochrysis carterae on melanogenesis in B16F1 and B16F10 melanoma cells. P. carterae extract inhibited the α-melanocyte-stimulating hormone (α-MSH)-enhanced melanin synthesis in B16F1 melanoma cells. P. carterae also inhibited unstimulated melanin synthesis in B16F10 melanoma cells. Western blotting showed that the P. carterae extract inhibited tyrosinase and microphthalmia-associated transcription factor (MITF) in a dose-dependent manner. The reporter assay also revealed a decline in the tyrosinase promoter activity in the presence of P. carterae extract. Furthermore, quantitative real-time RT-PCR analysis showed that P. carterae extract downregulated the mRNA levels of tyrosinase and MITF. Finally, our study demonstrated that the hot-water extract of P. carterae inhibits melanin synthesis via the down-regulation of MITF mRNA level. Our findings indicate that P. carterae extract could be a possible cosmetic ingredient.

Highlights

Coccolithophore (Haptophyta) are a group of unicellular marine microalgae that have unique calcium carbonate scales called coccoliths [1]
We attempted to clarify the effect of the coccolithophore alga P. carterae on melanin synthesis
B16F1 melanoma cells were cultured in the presence of P. carterae extract for 4 days, and cell growth was determined using the crystal violet assay

Summary

Introduction

Coccolithophore (Haptophyta) are a group of unicellular marine microalgae that have unique calcium carbonate scales called coccoliths [1]. The coccolithophore, Pleurochrysis carterae (Figure 1), is currently regarded as one of the most promising candidates for biodiesel production as it has a high lipid content [2]. The calcium-rich P. carterae has already been used as a calcium supplement [3]. Melanin has various important biological functions, including protection of the skin from ultraviolet light, and is one of the skin color determinants [4]. Tyrosinase is a rate-limiting enzyme and plays a crucial role in melanin synthesis under the control of various signals. CAMP-protein kinase A (PKA) dependent pathway is one of the most important pathways for activation of melanogenesis Tyrosinase is a rate-limiting enzyme and plays a crucial role in melanin synthesis under the control of various signals. cAMP-protein kinase A (PKA) dependent pathway is one of the most important pathways for activation of melanogenesis

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