Abstract
Pleomorphic adenoma gene 1 mediates the role of Karyopherin alpha 2 and has prognostic significance in hepatocellular carcinoma
Highlights
Karyopherin alpha 2 (KPNA2) promotes tumor growth in hepatocellular carcinoma (HCC)
Transcriptional factor pleomorphic adenoma gene 1 (PLAG1) is promoted into nucleus by KPNA2 We applied co-immunoprecipitation using a polyclonal antibody of KPNA2 and proteins acquired from the assays were used for detection of PLAG1, with ACTB as a negative control
The association of PLAG1 with KPNA2 was confirmed in two HCC cell lines, as PLAG1, but not ACTB, could be detected in the precipitate enriched by KPNA2 antibody (Figure 1a)
Summary
Karyopherin alpha 2 (KPNA2) promotes tumor growth in hepatocellular carcinoma (HCC). Plenty of researches have performed to explore mechanisms underlying the initiation, propagation and development of HCC [3,4]. It raised the hypothesis that KPNA2 might affect cancer cells through the translocation of cancer-associated transcriptional factors. Previous report has indicated the direct association of KPNA2 with a zinc-finger transcription factors pleomorphic adenoma gene 1 (PLAG1) by the yeast twohybrid system [13], suggesting PLAG1 might be one of critical mediators of KPNA2 effects in malignant diseases. Whether KPNA2 might associate with PLAG1 and assist PLAG1 nucleus import to activate downstream effectors in HCC remains unclassified. We explored the functional interaction of KPNA2 with PLAG1 and the clinical significance of the mechanism in HCC
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