Pleiotropic regulatory function of the RNA chaperone Hfq in the Pseudomonas protegens FD6

Abstract

The rhizosphere bacterium Pseudomonas protegens FD6, which is associated with the production of antibiotics such as pyoluteorin (PLT) and 2,4-diacetylphloroglucinol (2,4-DAPG), has strong antagonistic effects on phytopathogens. Hfq is a conserved RNA chaperone involved in the regulation of stress tolerance, antibiotic production, and bacterial virulence. Here, we determined the regulatory effects of Hfq on biocontrol traits in P. protegens FD6. Mutation of hfq in FD6 reduced the growth rate, swimming and swarming ability, and production of proteases, siderophores, and hydrogen cyanide (HCN). Transmission electron microscopy revealed that disruption of hfq led to the loss of flagella. Furthermore, Hfq exerted a suppressive effect on biofilm formation and PLT production while not affecting antagonistic activity and control effect against tomato bacterial wilt. However, Hfq positively regulated the production of 2,4-DAPG and activated the phlD expression. Additionally, mutation of hfq abolished the function of the type VI secretion system (T6SS) due to a decrease in the expression of T6SS-related genes. Overall, these results suggest that Hfq plays a pleiotropic role in modulating the expression of biocontrol trait-related genes in P. protegens FD6.