Pleionea sediminis sp. nov., isolated from coastal sediment and emendation of the description of the genus Pleionea.

Abstract

A Gram-stain-negative, rod-shaped and non-motile bacterium, designated strain S1-5-21T, was isolated from coastal sediment in Quanzhou Bay, PR China, and subjected to taxonomic characterization. Growth of strain S1-5-21T was observed at 15-40 °C (optimum, 30-35 °C), NaCl tolerance of 0.5-10 % NaCl (w/v; optimum, 1 %), and pH range of 5-8 (optimum, pH 7). Catalase activity and oxidase activity were found to be positive. The nearly complete 16S rRNA gene sequence of strain S1-5-21T shows maximum sequence similarity to Pleionea mediterranea MOLA115T (95.7 %), followed by Kangiella chungangensis CAU 1040T (90.4 %) and Aliikangiella marina GYP-15T (90.1 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S1-5-21T forms a distinct monophyletic branch affiliated to the genus Pleionea. The ANI value between strain S1-5-21T and close relative P. mediterranea MOLA115T was estimated to be 70.8 %. The respiratory quinone was found to be Q-8. The predominant fatty acids (>10 %) were identified as iso-C15 : 0, summed feature 9 (iso-C17 : 1 ω9c and/or C16 : 0 10-methyl) and iso-C16 : 0. The polar lipids were identified as phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two aminolipids, three phospholipids and five unidentified polar lipids. The draft genome size of strain S1-5-21T is 5.2 Mb with genomic G+C content of 40.1 mol%. Based on these results, strain S1-5-21T is concluded to represent a novel species within the genus Pleionea, for which the name Pleioneasediminis sp. nov. is proposed with the type strain S1-5-21T (=MCCC 1K03814T=KCTC 72149T).