Platelets aggregation under influence of immunoglobulin class G separated from the blood plasma of patients with ischemic stroke

Abstract

Ischemic stroke provoke irreversible changes in the organism and fully recovery was not observed. Some reactivity of the haemostasis system were shoved during the acute phase of ischemic stroke as well as post few year. The idea was explored that specific autoantibody generated in the bloodstream during the acute phase and their existence in bloodstream past one year could provoke disease repetition. Fractions of immunoglobulin class G were separated: from the blood plasma of healthy donors, patients with atherothrombotic and cardioembolic ischemic stroke in acute phase of disease and the analogical fractions from the same patients one year past acute phase. The influence of the separated fractions on the process of ADF-induced healthy donor's platelets aggregation was observed. It was showed that impact of immunoglobulin class G was characterized by one-wave irreversible ADP-induced platelet aggregation. The maximum aggregation was shoved under influence of IgG fraction separated from the patients with AIS. This influence was on the 15 % more intensive in comparison with influence of IgG fraction separated from the healthy donors. One year past disease all tested IgG fractions provoked inhibition of platelets aggregation up to 25 %. The maximum inhibition of ADP-dependent healthy donor's platelets aggregation was provided by fraction separated from the patients with AIS one year past acute phase.