Platelet-rich plasma induces mRNA expression of VEGF and PDGF in rat bone marrow stromal cell differentiation

Abstract

To investigate the potentially useful of platelet-rich plasma (PRP) on mRNA expression of angiogenesis. Adjunct assay and reverse-transcription polymerase chain reaction (RT-PCR) analysis of type I collagen, vascular endothelial growth factor (VEGF), and platelet-derived growth factor (PDGF) in rat bone marrow stromal cells differentiation in 14 days' culture. The PRP significantly elevated alkaline phosphatase activity after day 5 (P < .05), and DNA and protein content increased at culture days 1, 3, and 5 (P < .01) with PRP compared with control. The RT-PCR demonstrated that type I collagen was expressed in all substrates and remained high with PRP during 14 days of culture, and that mRNA expression of VEGF and PDGF were higher over time. This study indicates a potential contribution of PRP as possibly starting the process of angiogenesis, recruiting the endothelial cells which line blood vessels, and beginning the initiation of bone regeneration.