Platelet-Rich Fibrin Can Neutralize Hydrogen Peroxide-Induced Cell Death in Gingival Fibroblasts.

Zahra Kargarpour,Jila Nasirzade,Francesca Di Summa,Richard J Miron,Reinhard Gruber,Layla Panahipour

doi:10.3390/antiox9060560

Zahra Kargarpour, Jila Nasirzade + Show 4 more

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https://doi.org/10.3390/antiox9060560

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Journal: Antioxidants	Publication Date: Jun 26, 2020
Citations: 18	License type: CC BY 4.0

Affiliation: Medical University of Vienna, University of Bern

Abstract

Hydrogen peroxide is a damage signal at sites of chronic inflammation. The question arises whether platelet-rich fibrin (PRF), platelet-poor plasma (PPP), and the buffy coat can neutralize hydrogen peroxide toxicity and thereby counteract local oxidative stress. In the present study, gingival fibroblasts cells were exposed to hydrogen peroxide with and without lysates obtained from PRF membranes, PPP, heated PPP (75 °C for 10 min), and the buffy coat. Cell viability was examined by trypan blue staining, live-dead staining, and formazan crystal formation. Cell apoptosis was assessed by cleaved caspase-3 Western blot analysis. Reverse transcription-quantitative polymerase chain reaction (RT-PCR) was utilized to determine the impact of PRF lysates on the expression of catalase in fibroblasts. It was reported that lysates from PRF, PPP, and the buffy coat—but not heated PPP—abolished the hydrogen peroxide-induced toxicity in gingival fibroblasts. Necrosis was confirmed by a loss of membrane integrity and apoptosis was ruled out by the lack of cleavage of caspase-3. Aminotriazole, an inhibitor of catalase, reduced the cytoprotective activity of PRF lysates yet blocking of glutathione peroxidase by mercaptosuccinate did not show the same effect. PRF lysates had no impact on the expression of catalase in gingival fibroblasts. These findings suggest that PRF, PPP, and the buffy coat can neutralize hydrogen peroxide through the release of heat-sensitive catalase.

Highlights

Platelet-rich fibrin (PRF) is a fibrin-rich network that serves as a scaffold for bioactive molecules released from platelets being widely used in regenerative dentistry [1]
We analyzed the effects of PRF lysates on human gingival fibroblasts treated with or by the uptake of trypan blue which was not observed in the presence of PRF lysates (Figure 1A)
Hydrogen peroxide led to a rapid disruption of the cell membranes lysates inhibited acute ofnot hydrogen peroxide indicated by live-dead indicated by the uptake ofthe trypan bluetoxicity which was observed in the presence of PRF lysates (Figure staining lysates inhibited the acute toxicityprevented of hydrogen peroxide indicated by live-dead reduction (Figure 1A)

Summary

Introduction

Platelet-rich fibrin (PRF) is a fibrin-rich network that serves as a scaffold for bioactive molecules released from platelets being widely used in regenerative dentistry [1]. PRF has been shown to limit dimensional changes of the alveolar ridge post-extraction [2]. PRF has been utilized as an adjuvant for peri-implantitis therapy [3,4,5]. PRF has been utilized to promote the healing and wound closure of chronic ulcers [6] and resurfacing of full-thickness burns [7]. Apart from its antimicrobial activity [10], PRF can suppress inflammation [11] and osteoclastogenesis [12]

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