Platelet storage lesion: The limited impacts of leukoreduction processes in retention of microvesicules, prion, and platelet-derived biological response modifiers

Abstract

Currently, platelet concentrates are produced either from pooled buffy coats which are leukoreduced during processing by WBC removal filters, or several apheresis technologies with or without filtration. The effect of exposure to artificial surfaces during apheresis leukoreduction processes and the exposure to the blood collection set, filters and storage bags, on the generation/retention of microvesicles, prion protein and major biological response modifiers is largely unknown. Exposure to the matrix of charged platelet filters can lead to activation of complement, kallikrein/kinin and inflammatory systems as well as platelet activation, secretion and microvesiculation. It is therefore important to define the impact of various leukocyte removal processes on the extent of the platelet storage lesion and cellular apoptosis/necrosis.