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Abstract
Purpose Osteoarthritis (OA) is one of the common degenerative diseases of the joint in the world. This study was designed to explore the effect of platelet-rich plasma (PRP) combined with alendronate (ALN) on OA. Methods We induced OA model by anterior cruciate ligament transection (ACLT) method in rats and treating chondrocytes by IL-1β in vitro. PRP and/or ALN were used to treat induced rats and chondrocytes. Hematoxylin and eosin (H&E) and Safranin O staining were used to observe the structures of cartilage. The mRNA expression of Collagen II, MMP-13, and inflammatory factors (IL-18, IL-1β, and TNF-α) in the cartilage and chondrocytes of rats was determined by qRT-PCR. The expression of NF-κB pathway-related proteins (p-p65, p65, IκBα, and p-IκBα) in the cartilage and chondrocytes of rats was determined by Western blot. The proliferation of chondrocytes was detected by MTT assay. Results Treatment with PRP, ALN, or PRP combined with ALN decreased the degree of cartilage destruction, the mRNA expression of MMP-13 and inflammatory factors (IL-18, IL-1β, and TNF-α), and the protein expression of p-IκBα/IκBα and p-p65/p65, increased Collagen II expression, and the threshold of tender and thermal pain in OA rats. Meanwhile, ALN, PRP, or ALN combined with PRP reversed the inhibiting effect of phorbol myristate acetate (PMA, an NF-κB agonist) on cell proliferation and cartilage matrix metabolism. Among them, the effects of ALN combined with PRP were most obvious. Conclusion PRP combined with ALN delayed OA progression by inhibiting the NF-κB signaling pathway.
Highlights
Osteoarthritis (OA) is a kind of chronic joint disease that usually occurs in older people [1]
We found that platelet-rich plasma (PRP) combined with ALN delayed the progression of OA in rats through inhibiting inflammation and improving cartilage matrix metabolism
PRP combined with ALN promoted cell proliferation and improved cartilage matrix metabolism via inhibiting NF-κB signaling pathway in rat chondrocytes
Summary
Osteoarthritis (OA) is a kind of chronic joint disease that usually occurs in older people [1] It is characterized by joint stiffness, persistent pain, and even disability, accompanied by varying degrees of inflammation and defects in articular cartilage [2]. A research points out that more than 1/4 of the adult population is affected by OA, and the disease is the main cause of morbidity in people over 40 years old by the year 2020 [4] At present, it mainly relieves symptoms by injecting lubricating supplements into the joint cavity or using topical nonsteroidal anti-inflammatory drugs, steroids, physical treatments, and so on [5].
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