Abstract
Background: Interstitial cystitis/painful bladder syndrome (IC/PBS) could be treated to ameliorate urothelial injury. Here, we investigated the efficacy of intravesical instillation with platelet-rich plasma (PRP) and hyaluronic acid for acute IC/PBS. Methods: The effects of PRP and hyaluronic acid on the proliferation of normal human fibroblast cells (HFCs) were assessed. Additionally, thirty virgin female rats were randomized into five groups: group 1, saline-injected control; group 2, cyclophosphamide (CYP) plus intravesical instillation with normal saline; group 3, CYP plus intravesical instillation with hyaluronic acid (1 mg/mL); group 4, CYP plus intravesical instillation with PRP; and group 5, CYP plus intravesical instillation with PRP plus hyaluronic acid. A cystometry and histological assessments were performed. The expression of cell junction-associated protein zonula occludens-2 (ZO-2) and inflammatory cytokine interleukin 6 (IL-6) was also measured. Results: Low dose PRP increased proliferation in HFCs. The acute IC/PBS rats showed significantly lower voiding interval values. Voiding interval values were significantly higher in the CYP plus intravesical instillation with PRP group than in the CYP-induced acute IC/PBS group. Additionally, the expression of ZO-2 was increased and IL-6 was decreased in the CYP plus intravesical instillation with PRP group compared with the CYP-induced acute IC/PBS group. Conclusion: These findings suggest that PRP modulate urothelial repair, which ameliorate the increase in urination frequency in rats treated with CYP. Overall, PRP may confer potential benefits by acting as urothelial repair modulators.
Highlights
Interstitial cystitis/painful bladder syndrome (IC/PBS) is a chronic illness regarded as clinical by recurring events of pelvic pain and increased urination frequency [1,2], significantly damaging patients’quality of life
human fibroblast cells (HFCs) were treated with various concentrations of platelet-rich plasma (PRP) (1–10%) for 24 h and cell proliferation was quantified by MTT assay
We observed that urothelial thickness decreased in the bladder of rats with acute IC/PBS. These findings suggested that damage to the urothelium might lead to typical signs of a leaky barrier, which may result in an increased urination frequency in acute IC/PBS rats
Summary
Interstitial cystitis/painful bladder syndrome (IC/PBS) is a chronic illness regarded as clinical by recurring events of pelvic pain and increased urination frequency [1,2], significantly damaging patients’quality of life. Interstitial cystitis/painful bladder syndrome (IC/PBS) is a chronic illness regarded as clinical by recurring events of pelvic pain and increased urination frequency [1,2], significantly damaging patients’. In light of the limited availability of human tissue for study, animal models are an imperative adjunct in improving our comprehension of IC/PBS. Bladder inflammation caused by a single intraperitoneal injection of cyclophosphamide (CYP) in rodents is a commonly used noninvasive model of acute IC/PBS [6,7]. Interstitial cystitis/painful bladder syndrome (IC/PBS) could be treated to ameliorate urothelial injury. We investigated the efficacy of intravesical instillation with platelet-rich plasma (PRP) and hyaluronic acid for acute IC/PBS. Methods: The effects of PRP and hyaluronic acid on the proliferation of normal human fibroblast cells (HFCs) were assessed. The expression of cell junction-associated protein zonula occludens-2 (ZO-2) and inflammatory cytokine interleukin 6
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