Abstract
BackgroundReported efficacy of platelet-rich plasma (PRP) in regenerative medicine is contradictory. We validated the effects of PRP on proliferation of canine bone marrow-derived multipotent mesenchymal stromal cells (K9BMMSCs) in vitro. PRP was extracted from blood of six dogs with osteoarthritis. K9BMMSCs were established from bone marrow and characterized for CD90 and CD19 expression by immunocytochemistry. Effects of PRP concentrations on viability of matching autologous K9BMMSCs were validated using MTS assay.ResultsPositive CD90 and negative CD19 expression confirmed MSC origin. PRP at 40% volume/volume concentration increased, while PRP at 80 and 100% v/v concentrations suppressed viability of tested K9BMMSCs.ConclusionPRP concentration plays an important role in K9BMMSCs viability, which could affect tissue repairs in vivo.
Highlights
Reported efficacy of platelet-rich plasma (PRP) in regenerative medicine is contradictory
We have evaluated the effects of PRP concentration on the cell viability of the autologous canine bone-marrow derived multipotent mesenchymal stromal cells (K9BMMSCs) harvested from client-owned dogs with a history of OA in vitro
Isolation and characterization of Canine bone marrow-derived multipotent mesenchymal stromal cells (K9BMMSC) cells We successfully isolated K9BMMSC cells from six dogs diagnosed with OA (Table 1)
Summary
Reported efficacy of platelet-rich plasma (PRP) in regenerative medicine is contradictory. Platelet-rich plasma (PRP) is an enriched plasma containing variety of growth factors, including the platelet derived growth factor (PDGF), vascular endothelial growth factor (VEGF), transforming growth factor-β (TGF-β), fibroblast growth factor (FGF), and insulin-like growth factors I and II (IGF-I, IGF-II) [1, 2] These growth factors are potent chemoattractant and mitogens, which help attract and activate surrounding cells at sites of injury. A consensus on the actual benefits of PRP has not yet been established Such variation in outcomes related to PRP treatment could be attributed to some aspects of study design, such as sample sizes and control selections, in addition to the type of disease under investigation [1]. Another contributing factor could be the concentration and volume of PRP used during these treatments
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
