Platelet-rich fibrin prepared from stored whole-blood samples

Kazushige Isobe,Masayuki Nakamura,Hideo Kawabata,Toshimitsu Okudera,Tomoyuki Kawase,Takaaki Tanaka,Yutaka Kitamura,Taisuke Watanabe,Masashi Suzuki,Taiji Suzuki,Kohya Uematsu,Koh Nakata,Hajime Okudera

doi:10.1186/s40729-017-0068-4

Abstract

BackgroundIn regenerative therapy, self-clotted platelet concentrates, such as platelet-rich fibrin (PRF), are generally prepared on-site and are immediately used for treatment. If blood samples or prepared clots can be preserved for several days, their clinical applicability will expand. Here, we prepared PRF from stored whole-blood samples and examined their characteristics.MethodsBlood samples were collected from non-smoking, healthy male donors (aged 27–67 years, N = 6), and PRF clots were prepared immediately or after storage for 1–2 days. Fibrin fiber was examined by scanning electron microscopy. Bioactivity was evaluated by means of a bioassay system involving human periosteal cells, whereas PDGF-BB concentrations were determined by an enzyme-linked immunosorbent assay.ResultsAddition of optimal amounts of a 10% CaCl2 solution restored the coagulative ability of whole-blood samples that contained an anticoagulant (acid citrate dextrose) and were stored for up to 2 days at ambient temperature. In PRF clots prepared from the stored whole-blood samples, the thickness and cross-links of fibrin fibers were almost identical to those of freshly prepared PRF clots. PDGF-BB concentrations in the PRF extract were significantly lower in stored whole-blood samples than in fresh samples; however, both extracts had similar stimulatory effects on periosteal-cell proliferation.ConclusionsQuality of PRF clots prepared from stored whole-blood samples is not reduced significantly and can be ensured for use in regenerative therapy. Therefore, the proposed method enables a more flexible treatment schedule and choice of a more suitable platelet concentrate immediately before treatment, not after blood collection.

Highlights

In regenerative therapy, self-clotted platelet concentrates, such as platelet-rich fibrin (PRF), are generally prepared on-site and are immediately used for treatment
We developed a method for preparation of PRF from stored WB samples by adding CaCl2 and evaluated the quality in terms of suitability for regenerative therapy
Because glucose is contained in the Acid citrate dextrose solution (ACD)-A solution, glucose levels in the stored WB and serum samples after centrifugation were significantly greater than those of freshly collected WB samples

Summary

Introduction

Self-clotted platelet concentrates, such as platelet-rich fibrin (PRF), are generally prepared on-site and are immediately used for treatment. In Niigata University Hospital, when relatively severe surgical operations (e.g., large bone defects that require hospitalization for alveolar ridge augmentation and sinus floor elevation) are planned, relatively large volumes of blood samples are usually collected the day before the operation, and platelet-rich plasma (PRP) is prepared and stored at ambient temperature until use [5]. There are no established methods for preparation of self-clotted platelet concentrates from stored wholeblood (WB) samples. This may be another reason why platelet-rich fibrin (PRF) should be prepared on-site and used immediately

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Discussion

Conclusion