Abstract
BackgroundMesenchymal stem cells are promising candidates in regenerative cell therapy. Conventional culture methods involve the use of animal substances, specifically fetal bovine serum as growth supplement. Since the use of animal-derived products is undesirable for human applications, platelet lysates produced from human platelets are an attractive alternative. This is especially true if platelet lysates from already approved transfusion units at blood banks can be utilized. The purpose of this study was to produce human platelet lysates from expired, blood bank-approved platelet concentrates and evaluate their use as growth supplement in the culture of mesenchymal stem cells.Methodology/Principal FindingsIn this study, bone marrow-derived mesenchymal stem cells were cultured with one of three culture supplements; fetal bovine serum, lysates from freshly prepared human platelet concentrates, or lysates from expired human platelet concentrates. The effects of these platelet-derived culture supplements on basic mesenchymal stem cell characteristics were evaluated. All cultures maintained the typical mesenchymal stem cell surface marker expression, trilineage differentiation potential, and the ability to suppress in vitro immune responses. However, mesenchymal stem cells supplemented with platelet lysates proliferated faster than traditionally cultured cells and increased the expression of the osteogenic marker gene RUNX-2; yet no difference between the use of fresh and expired platelet concentrates was observed.Conclusion/SignificanceOur findings suggest that human platelet lysates produced from expired platelet concentrates can be used as an alternative to fetal bovine serum for mesenchymal stem cell culture to the same extent as lysates from fresh platelets.
Highlights
Platelets are small anucleated structures of hematopoietic origin that contribute to hemostasis and wound healing by secreting growth factors and cytokines
Conclusion/Significance: Our findings suggest that human platelet lysates produced from expired platelet concentrates can be used as an alternative to fetal bovine serum for mesenchymal stem cell culture to the same extent as lysates from fresh platelets
mesenchymal stem cells (MSC) were cultured in media supplemented with one of three different media supplements; fetal bovine serum (FBS), human platelet lysates (HPL) from fresh platelet concentrates (HPLF), and HPL derived from expired platelet concentrates (HPLO) for the total of three passages prior to analysis
Summary
Platelets are small anucleated structures of hematopoietic origin that contribute to hemostasis and wound healing by secreting growth factors and cytokines. Keeping the physiological levels of platelets well balanced is essential since quantitative and qualitative platelet disorders can be severe and difficult to treat, affecting the patient’s ability to respond to bleeding and form adequate platelet plugs [2] Due to their importance, platelet contribution to hemostasis and biology has been thoroughly evaluated in numerous studies in the past [3,4]. The purpose of this study was to produce human platelet lysates from expired, blood bank-approved platelet concentrates and evaluate their use as growth supplement in the culture of mesenchymal stem cells
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