Abstract
ABSTRACTPEAR1 is highly expressed at bovine MDSC differentiation. However, its biological function remains unclear. Western blotting results showed that PEAR1 increased between day 0 and day 2 of cell differentiation and decreased from day 3. Moreover, scratch test showed that wound healing rate increased after PEAR1 overexpression and decreased upon its suppression. Meanwhile, we found that, upon PEAR1 induction, both the expression of the focal adhesion-associated and MyoG, and the myotube fusion rate increased. However, when PEAR1 was suppressed, opposite results were obtained. Immunoprecipitation revealed an association between PEAR1 and ITGB1. Notably, inhibition of FAK and ITGB1 repressed cell differentiation. In conclusion, our study indicated that PEAR1 is involved in the regulation of bovine MDSC migration and differentiation.
Highlights
Platelet endothelial aggregation receptor-1 (PEAR1; known as JEDI or MEGF12) is a type-1 transmembrane protein of the multiple epidermal growth factor – like domain protein family
Immunofluorescence results showed the presence of PEAR1 signal during bovine MDSC differentiation (Figure 1(e))
In a previous study analyzing PEAR1 transcription during MDSC differentiation, we reported that PEAR1 expression gradually increased from day 0
Summary
Platelet endothelial aggregation receptor-1 (PEAR1; known as JEDI or MEGF12) is a type-1 transmembrane protein of the multiple epidermal growth factor – like domain protein family. PEAR1 is mainly expressed in platelets and endothelial cells, as well as satellite glial cell precursors [2]. DxS promotes platelet aggrega – tion by directly phosphorylating PEAR1 to activate PI3K/Akt [3]. During development of the peripheral nervous system, PEAR1 promotes phagocytosis through a nonclassical phosphor – ylation-dependent mechanism [4]. Knockdown of PEAR1 doubled the proliferation rate and significantly stimulated cell migration, which enhanced in vitro tube formation on matrigel through the Akt/PTEN-dependent p21/CDC2 pathway. Overexpression of PEAR1 in NIH 3T3 fibroblasts resulted in a reduction of late and early myeloid progenitors in non-adherent co-cultured bone marrow cells, suggesting that PEAR1 is involved in the early stages of hematopoietic differentiation [5]
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