Platelet derived growth factor receptor α-phospholipase Cγ1-protein kinase Cα signal transductionin in human lung cancer cell GLC-82 for combination of the drugs tumor necrosis factor-related apoptosis-inducing ligand and proteasome inhibitor MG132

Abstract

Objective To tumor necrosis factor- related apoptosis- inducing ligand(TRAIL)combined with a protease inhibitor MG132 observed in human lung adenocarcinoma cell line GLC- 82 inhibition of proliferation and platelet derived growth factor receptor α(PDGFRα)- phospholipase Cγ1(PLCγ1)-protein kinase Cα(PKCα)signal transduction pathway. Methods Methyl thiazol tetrazolium(MTT)assay inhibition of TRAIL combined with a protease inhibitor MG132 on GLC- 82 cell growth detection; using flow cytometry to detect the effects of TRAIL combined with a protease inhibitor MG132 on GLC- 82 cell apoptosis, using SP immunohistochemical detection of protease inhibitors MG132 joint expression of TRAIL- associated protein PDGFRα, PLCγ1,PKCα's, Western blotting to determine the effect of protease inhibitors MG132 TRAIL combined for GLC- 82 cells PDGFRα, PLCγ1, PKCαprotein expression. Results MTT assay inhibition of TRAIL combined with a protease inhibitor MG132 on GLC- 82 cell growth detection. The inhibitory rates were(34.92±3.95)%,(33.17±1.78)% and(76.96±1.28)%, respectively; using flow cytometry to detect the effects of TRAIL combined with a protease inhibitor MG132 on GLC- 82 cell apoptosis. Apoptosis rates of the four group were 6.78%, 38.72%, 45.60% and 91.74%, respectively. Using SPimmunohistochemical detection of protease inhibitors MG132 joint expression of TRAIL- associated protein PDGFRα, PLCγ1, PKCα's, Western blotting to determine the effect of protease inhibitors MG132 TRAIL combined for GLC- 82 cells PDGFRα, PLCγ1, PKCα protein expression. Conclusion TRAIL combined protease inhibitor MG132 on human lung adenocarcinoma GLC- 82 cells significantly inhibited the growth, can reduce the level of GLC- 82 cells PDGFRα- PLCγ1- PKCα each protein signaling pathway, the incidence of lung cancer and PDGFRα- PLCγ1- PKCα enhanced signal transduction pathways are closely related. Key words: Tumor necrosis factor-related apoptosis-inducing ligand; MG132; Lung adenocarcinoma; Signal transduction