Abstract
ABSTRACTPurposeHerein we evaluated the effects of platelet concentrate (PC) and platelet-poor plasma (PPP) on bone repair using noncritical defects in the calvaria of rabbits and compared them to the presence of TGF-β1 and osteocalcin on reparative sites.MethodsFive noncritical defects of 8.7 mm in diameter were created on the calvaria of 15 animals. Each defect was treated differently, using autograft (ABG), ABG associated with PC (ABG + PC), ABG with PPP (ABG + PPP), isolated PPP, and blood clot (control). The animals were submitted to euthanasia on the second, fourth and sixth week post-surgery.ResultsThe defects that received ABG+PC or PPP demonstrated lower bone formation when compared to specimens that received ABG in the same period. These results coincided to significant higher immunopositivity for TGF-β1 for specimens that received PC, and lower presence of cytokine in the group PPP. However, either higher or lower presence of TGF-β1 were also correlated to lower presence of osteocalcin. Likewise, these results were similar to findings in specimens treated only with PPP when compared to control.ConclusionsPC and PPP were not effective when applied in association with ABG. Similarly, isolated use of PPP was not beneficial in optimizing the bone repair.
Highlights
It is an agreement in the literature that the use of autogenous particulate bone graft (ABG) has been considered the gold standard among graft materials for bone repair in craniofacial bone[1]
Purpose: we evaluated the effects of platelet concentrate (PC) and platelet-poor plasma (PPP) on bone repair using noncritical defects in the calvaria of rabbits and compared them to the presence of TGF-β1 and osteocalcin on reparative sites
We used the non-critical model created in rabbit calvaria, maintaining the cortical bone to assess the osteogenic effects of PC and PPP
Summary
It is an agreement in the literature that the use of autogenous particulate bone graft (ABG) has been considered the gold standard among graft materials for bone repair in craniofacial bone[1]. The premise about the use of PC on wound healing tissue is associated to the presence of platelets, that when activated release several amounts of growth factors that include PDGF, TGF-b, VEGF, IGF-1, and EGF4-6. These growth factors, especially the TGF-b, may contribute to stem cells’ mesenchymal chemotaxis, synthesis, and secretion of collagen on extracellular matrix, as well as to proliferation and cell differentiation into osteoblast, increasing the expression of osteocalcin, favoring bone tissue regeneration and osteoconduction[7,8]. Previous studies were not able to identify osteogenic action of PC when it was used alone to repair bone defects[7,9]
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