Abstract

It is of interest to refine the taxonomic status of C. melo ssp. agrestis using its plastome data. The chloroplast size and GC% was found to be 1,56,016 bp and 36.92% respectively in Cucumis melo subsp. agrestis. The plastome of C. melo subsp. agrestis comprises of two inverted repeat (IR) regions of 25,797 bp each. It consisted of 133 genes with 88 protein-coding genes, 8 rRNA genes and 37 tRNA genes. Analysis of the C. melo ssp. agrestis plastome data will help breeders to improve the yield the crop.

Highlights

  • The cucurbits are vegetable crops of the Cucurbitaceae family

  • Results & Discussion: The plastome of C. melo subsp. agrestis comprise (Figure 3) of 1,56,016 bp circular DNA carrying 25,797 bp each of two inverted repeat (IR) regions, divided by large single-copy (LSC) regions of 86,335 bp and small single-copy (SSC) regions of 18,088 bp, respectively. It contained a GC% of 36.92, a total number of 133 genes, including 88 protein-coding genes, 37 tRNA genes, and eight rRNA genes. This data is consistent with Cucumis hystrix, Cucumis melo, Cucumis sativus

  • The plastome size varies from 1,59,232 bp in Gerrardanthus macrorhizus to 1,47,874 bp in Linnaeosicyos amara, the differences of GC% was recorded in the range of 36 to 37%, Ampelosycios humblotii was recorded with minimum 79 CDS, while maximum 88 CDS was recorded in Trichosanthes wallichiana (Figure 4)

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Summary

Background

The cucurbits are vegetable crops of the Cucurbitaceae family. This family carries total 98 genera and 1000 species. Muskmelon (Cucumis melo L.) is a member of the family Cucurbitaceae. The genus Cucumis possess large phenotypic diversity with C. hystrix, C. callosus, and C. sativus var. A number of cucurbits including Cucumis melo subsp. Agrestis are used in the indigenous system of medicine. Cucurbitacins in cucurbits possess renowned biological attributes [2]. Information on the reproductive biology of Cucumis melo subsp. Agrestis is known [14]. It is of interest to refine the taxonomic status of C. melo ssp. Agrestis using generation sequencing (NGS) plastome data for further application [3] It is of interest to refine the taxonomic status of C. melo ssp. agrestis using generation sequencing (NGS) plastome data for further application [3]

Materials and Methods
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