Abstract
AbstractRNA interference (RNAi) technology is a promising and effective approach for pest insect management. Owing to its sequence‐guided working mechanism, RNAi has a high degree of species‐selectivity, thus minimizing potential adverse effects on nontarget organisms. Recently, engineering plastid (chloroplast) genome, rather than the nuclear genome, to produce double‐stranded RNAs has emerged as a powerful way to protect plants from multiple arthropod pests. Here, we review the recent progresses in the plastid‐mediated RNAi (PM‐RNAi) approach for pest control and the factors influencing its efficacy, and propose the strategies for further efficiency improvement. We also discuss the current challenges and the biosafety‐related issues of PM‐RNAi technology that need to be addressed for commercial production.
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