Abstract
Etioplast development and protochlorophyllide (Pchlide) accumulation was studied in wheat seedlings (Triticum aestivum L. cv. Walde, Weibull) grown in darkness on gibberellic acid (GA3), gabaculine (3‐amino‐2,3‐dihydrobenzoic acid), or on a combination of the two. The results were compared with the features of seedlings grown on water only. GA3 enhanced shoot growth and promoted etioplast development. A correlation was observed between the appearance of prolamellar bodies (PLBs) and of phototransformable Pchlide. Gabaculine, a known tetrapyrrole biosynthesis inhibitor, delayed growth, slowed down the rate of PLB formation and caused structural alterations of the etioplasts up to 48 h of germination. Gabaculine also delayed the formation of phototransformable Pchlide as well as overall Pchlide biosynthesis, as determined by low‐temperature fluorescence emission in vivo. The spectral blue‐shift of newly formed chlorophyllide (Chlide) was delayed in irradiated dark‐grown gabaculine‐grown seedlings, indicating an inhibited dissociation of Chlide and NADPH‐Pchlide oxidoreductase (Pchlide reductase: EC 1.3.1.33). Thus there is a close correlation between accumulation of Pchlide and etioplast development, also under conditions when development is enhanced or delayed.
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