Abstract
Probing the binding between a microbe and surface is critical for understanding biofilm formation processes, developing biosensors, and designing biomaterials, but it remains a challenge. Here, we demonstrate a method to measure the interfacial forces of bacteria attached to the surface. We tracked the intrinsic fluctuations of individual bacterial cells using an interferometric plasmonic imaging technique. Unlike the existing methods, this approach determined the potential energy profile and quantified the adhesion strength of single cells by analyzing the fluctuations. This method provides insights into biofilm formation and can also serve as a promising platform for investigating biological entity/surface interactions, such as pathogenicity, microbial cell capture and detection, and antimicrobial interface screening.
Highlights
Probing the binding between a microbe and surface is critical for understanding biofilm formation processes, developing biosensors, and designing biomaterials, but it remains a challenge
Upon bacterial attachment to the surface, weak interactions occurred between the surface and cell envelope, which allowed the vibration of the bacterial cell [5, 13, 14]
We present an optical imaging method that is able to measure the adhesion strength of single microbial cells
Summary
Probing the binding between a microbe and surface is critical for understanding biofilm formation processes, developing biosensors, and designing biomaterials, but it remains a challenge. We tracked the intrinsic fluctuations of individual bacterial cells using an interferometric plasmonic imaging technique. This approach determined the potential energy profile and quantified the adhesion strength of single cells by analyzing the fluctuations. This method provides insights into biofilm formation and can serve as a promising platform for investigating biological entity/surface interactions, such as pathogenicity, microbial cell capture and detection, and antimicrobial interface screening. | | | microbial adhesion plasmonic imaging interfacial force biofilm formation binding, which were essential properties in understanding microbial adhesion. The knowledge obtained can help understand biofilm formation and be used in the design of artificial surfaces to minimize or maximize bacterial adhesion
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