Abstract
Surface enhanced Raman spectroscopy (SERS) is capable of sensing not only the chemical nature but also the biological organisms. In this work, it is experimentally demonstrated that silver and reduced graphene oxide nano-composite based material can be used as SERS platform. Localized surface plasmon resonance (LSPR) property of silver nanostructure such as nano-sphere, nano-dendrites and nano-prism and high impermeability of reduced graphene oxide (RGO) have been employed to build a composite for SERS measurements. Graphene oxide was synthesized using Hummer’s Method and then simple facile method was used for its reduction as well as for the deposition of silver nanostructure on it. SEM, XRD, FTIR, and UV-Vis techniques characterized the prepared nano-composite of Ag-RGO. The composite material was used for SERS measurement for the detection of Escherichia Coli (E. Coli) bacteria at a concentration of 8×106 CFU/ ml and it is observed that signal of silver nanodentries-RGO composite was intense in comparison with Ag-RGO nano-sphere and nano-triangular composite.
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