Plasmonic Nanohybrid with High Photothermal Conversion Efficiency for Simultaneously Effective Antibacterial/Anticancer Photothermal Therapy.

Abstract

Plasmonic metal/semiconductor nanohybrids hold great promise in photocatalysis and biosensor development; however, their potential phototherapeutic applications are yet fully unexplored. On the other hand, the demand of high laser power density to induce antibacterial photothermal therapeutic effects greatly restricts the practical applicability of the previously developed photothermal nanoagents (PTAs) for anticancer photothermal therapy (PTT). Here, we develop a plasmonic nanohybrid by integrating plasmonic noble metal gold nanorods (AuNRs) with a two-dimensional graphene oxide (2-D GO), capable to perform photothermal ablation of both bacterial pathogens as well as tumor cells, respectively, under low power single near-infrared (NIR) laser activation. Owing to the synergistic plasmonic photothermal effect (PPTT) of dual plasmonic PTAs, the plasmonic AuNR/GO nanohybrid exhibits remarkably higher photothermal conversion efficiency (PCE, 72.59%) than either individual AuNRs or GO under low laser power density (300 mW), leading to enhanced antibacterial/anticancer PTT. In addition, the synergistic plasmonic antibacterial/anticancer PTT induced by the plasmonic nanohybrid is also far superior to individual PTAs (AuNRs or GO), whereas the flow cytometric analysis of heat shock proteins (HSP 70) clearly dictates that the substantial killing of bacterial pathogens/tumor cells is solely due to the synergistic PPTT. Thus, the plasmonic AuNR/GO nanohybrid is a standalone PTA to perform simultaneous antibacterial/anticancer PTT under low power NIR laser activation for only 5 min, without any systemic side effects. The present study provides a clear demonstration about the potential therapeutic impact of plasmonic nanohybrids and thus will surely pave the way to design other hybrid nanoagents with enhanced PCE and integrate them with chemotherapeutic agents, leading to dual-modal chemo-/photothermal antibacterial/anticancer therapy under low power single laser excitation for a short duration.