Plasmonic Manipulation of DNA using a Combination of Optical and Thermophoretic Forces: Separation of Different-Sized DNA from Mixture Solution

Tatsuya Shoji,Tomohiro Yokoyama,Yasuyuki Tsuboi,Yuto Yamada,Kenta Itoh,Takahiro Yoshii,Noboru Kitamura,Junki Saitoh,Hajime Ishihara,Kei Murakoshi

doi:10.1038/s41598-020-60165-5

Tatsuya Shoji, Tomohiro Yokoyama + Show 8 more

Open Access

https://doi.org/10.1038/s41598-020-60165-5

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Abstract

We demonstrate the size-dependent separation and permanent immobilization of DNA on plasmonic substrates by means of plasmonic optical tweezers. We found that a gold nanopyramidal dimer array enhanced the optical force exerted on the DNA, leading to permanent immobilization of the DNA on the plasmonic substrate. The immobilization was realized by a combination of the plasmon-enhanced optical force and the thermophoretic force induced by a photothermal effect of the plasmons. In this study, we applied this phenomenon to the separation and fixation of size-different DNA. During plasmon excitation, DNA strands of different sizes became permanently immobilized on the plasmonic substrate forming micro-rings of DNA. The diameter of the ring was larger for longer DNA (in base pairs). When we used plasmonic optical tweezers to trap DNA of two different lengths dissolved in solution (φx DNA (5.4 kbp) and λ-DNA (48.5 kbp), or φx DNA and T4 DNA (166 kbp)), the DNA were immobilized, creating a double micro-ring pattern. The DNA were optically separated and immobilized in the double ring, with the shorter sized DNA and the larger one forming the smaller and larger rings, respectively. This phenomenon can be quantitatively explained as being due to a combination of the plasmon-enhanced optical force and the thermophoretic force. Our plasmonic optical tweezers open up a new avenue for the separation and immobilization of DNA, foreshadowing the emergence of optical separation and fixation of biomolecules such as proteins and other ncuelic acids.

Highlights

Where α is the polarizability of the micro/nano-particle to be trapped
In 2013, we demonstrated that plasmonic optical trapping (POT) of λ-DNA using a continuous-wave (CW) laser beam (λ = 808 nm) could be used to permanently fix DNA onto a plasmonic substrate[34]
To clarify and get a good understanding of POT, we have quantitatively evaluated the local rise in temperature (ΔT) and the thermophoretic force in POT for a gold nano-pyramidal dimer array by means of fluorescence correlation spectroscopy, and revealed that the thermophoretic force is never negligible and can significantly compete with the plasmon-enhanced optical force[32]

Summary

Introduction

Where α is the polarizability of the micro/nano-particle to be trapped. In the vicinity of the metallic nanostructure (in many cases it is a nanogap), E is highly localized around the nanogap, resulting in a huge enhancement in ∇E2. To clarify and get a good understanding of POT, we have quantitatively evaluated the local rise in temperature (ΔT) and the thermophoretic force (ft) in POT for a gold nano-pyramidal dimer array by means of fluorescence correlation spectroscopy, and revealed that the thermophoretic force is never negligible and can significantly compete with the plasmon-enhanced optical force[32]. Such thermal forces presumably limit the future development of POT. Based on the fact that the optical and thermophoretic forces are sensitive to particle size, we examined the possibility of optical separation and fixation of two different-sized DNA from mixture solution (5.4 kbp DNA and λ-DNA, or 5.4 kbp DNA and 166 kbp DNA)

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Results

Conclusion