Abstract
Background A newly published genus-specific PCR targeting mitochondrial genome was highly sensitive and specific (Haanshuus et al. 2013). The aim of the study was to decrease the laboratory time without impairing sensitivity or specificity, by converting the conventional PCR to real-time PCR using SYBR dye. A second aim was to include species-specific assays to be optionally run together with the genus-specific real-time assay.
Highlights
A newly published genus-specific PCR targeting mitochondrial genome was highly sensitive and specific (Haanshuus et al 2013)
Plasmodium genus- and species-specific real-time PCR using SYBR dye decreases laboratory time without impairing the sensitivity or specificity compared to conventional PCR
All the positives and the dilution series were analyzed in duplicates
Summary
A newly published genus-specific PCR targeting mitochondrial genome was highly sensitive and specific (Haanshuus et al 2013). The aim of the study was to decrease the laboratory time without impairing sensitivity or specificity, by converting the conventional PCR to real-time PCR using SYBR dye. A second aim was to include species-specific assays to be optionally run together with the genus-specific real-time assay
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