Abstract
Duraisingh, M. T., Curtis, J., and Warhurst, D. C. 1998.Plasmodium falciparum:Detection of polymorphisms in theDihydrofolate reductaseanddihydropteroate synthetasegenes by PCR and restriction digestion.Experimental Parasitology89,1–8. With the spread of resistance to chloroquine, the combination of sulphadoxine and pyrimethamine is growing in importance for the treatment of infection with the malaria parasitePlasmodium falciparum. Mutations in thedhfrgene ofP. falciparumhave been associated with resistance to pyrimethamine. Recently, several polymorphisms have been identified in theP. falciparum dhpsgene which may correlate with sulphadoxine-resistance. Simple and rapid tests have been developed to detect these polymorphisms, using PCR followed by restriction digestion. These tests can accurately identify all the polymorphisms described to date at codons 16, 51, 59, 108, and 164 in thedhfrgene and those at codons 436, 437, 540, 581, and 613 in thedhpsgene. A nested system has been developed which allows the accurate detection of these polymorphisms in samples of fingerprick blood collected on glass fiber membranes and filter papers, some with very low parasitaemias.
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