Abstract
The aim of this study was to determine if the addition of transferrin-selenium-ethanolamine (TSX) and/or plasminogen to mouse embryo culture medium would increase the incidence of blastocyst hatching and cell number. TSX is a commonly used cell culture supplement. Transferrin regulates iron uptake, selenium is a precursor for proteins acting as antioxidants, and ethanolamine supports cell membrane function. Plasminogen is a precursor to the protease plasmin that degrades fibrin and is thought to be a component of blastocyst hatching mechanisms in vivo.
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