Abstract
Plasmid profile analysis and curing of multidrug-resistant bacteria isolated from hospital environment in Akure metropolis, Ondo State Nigeria were investigated. Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Bacillus cereus were the bacterial isolated in the course of the research. The result revealed that Staphylococcus aureus and Pseudomonas aeruginosa showed multiple antibiotics resistance. Staphylococcus aureus and Pseudomonas aeruginosa are rapidly increasing as multidrug resistant strains worldwide. The results of antibiotics sensitivity test on the microorganisms subjected to plasmid analysis indicated that they were more susceptible to antibiotics after the plasmid curing of the microorganisms. The results from this investigative study have shown that resistance in S. aureus and P. aeruginosa are plasmid based as a result of its loss after curing.
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