Plasma Treatment of Fish Cells: The Importance of Defining Cell Culture Conditions in Comparative Studies

Henrike Rebl,Alexander Rebl,Claudia Bergemann,Barbara Nebe,Sebastian Rakers

doi:10.3390/app11062534

Abstract

The present study provides the fundamental results for the treatment of marine organisms with cold atmospheric pressure plasma. In farmed fish, skin lesions may occur as a result of intensive fish farming. Cold atmospheric plasma offers promising medical potential in wound healing processes. Since the underlying plasma-mediated mechanisms at the physical and cellular level are yet to be fully understood, we investigated the sensitivity of three fish cell lines to plasma treatment in comparison with mammalian cells. We varied (I) cell density, (II) culture medium, and (III) pyruvate concentration in the medium as experimental parameters. Depending on the experimental setup, the plasma treatment affected the viability of the different cell lines to varying degrees. We conclude that it is mandatory to use similar cell densities and an identical medium, or at least a medium with identical antioxidant capacity, when studying plasma effects on different cell lines. Altogether, fish cells showed a higher sensitivity towards plasma treatment than mammalian cells in most of our setups. These results should increase the understanding of the future treatment of fish.

Highlights

cold atmospheric pressure plasma (CAP) has been demonstrated to be beneficial for wound healing processes by reducing the microbial load, suppressing inflammatory events, reducing oxidative stress, and enhancing angiogenesis [1,2,3,4] in human and other vertebrate models [5,6]
Cell viability served as the criterion to evaluate whether fish cells react to plasma treatment with a similar sensitivity as mammalian cells
We found that the plasma treatment of fish cells requires lower dosages than of mammalian cells

Summary

Introduction

CAP has been demonstrated to be beneficial for wound healing processes by reducing the microbial load, suppressing inflammatory events, reducing oxidative stress, and enhancing angiogenesis [1,2,3,4] in human and other vertebrate models [5,6]. These CAP-induced benefits support the natural regenerative capacity of the integument, but have not yet been tested on aquatic organisms

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