Abstract
Abstract Data on the values of selected blood antioxidant parameters, i.e. total antioxidant capacity, glutathione peroxidase, and superoxide dismutase in healthy dogs, are lacking. There are no published accepted standard reference methods for their determination. The aim of this study was to determine the values of plasma total antioxidant capacity and the activities of whole blood glutathione peroxidase and erythrocyte superoxide dismutase in 30 healthy client-owned dogs (19 females, 11 males). The effect of age and sex on the measured antioxidant parameters was also investigated. Antioxidant parameters were determined with an automated biochemical analyser, using the commercially available Randox kits. No significant difference in age, weight, and antioxidant parameters was determined between females and males. A significant positive effect of age (p = 0.002, r2 = 0.284) on superoxide dismutase activity was confirmed. There was no effect of sex on any of the antioxidant parameters measured. However, we observed a tendency of the effect of sex (p = 0.063, r2 = 0.118), as well as age (p = 0.073, r2 = 0.111), on the activity of glutathione peroxidase. Our results are in part comparable with the results of other studies in which the same types of methods and samples were used to determine antioxidant parameters. In conclusion, the sex and age of dogs should be taken into consideration when planning a study on antioxidant status parameters.
Highlights
Increasing evidence indicates that oxidative stress significantly impairs the function of organs and plays a major role in the aetiology and pathogenesis of several diseases in humans and animals [1,2]
Tomsič et al: Plasma total antioxidant capacity and activities of blood glutathione peroxidase and superoxide dismutase determined in healthy dogs by using commercially available kits the overall antioxidant status of plasma and body fluids
The aim of this study was to determine the values of plasma Total antioxidant capacity (TAC) and activities of whole blood GSH-Px and erythrocyte superoxide dismutase (SOD) in clinically healthy dogs using commercially available kits applied to an automated biochemical analyser
Summary
Increasing evidence indicates that oxidative stress significantly impairs the function of organs and plays a major role in the aetiology and pathogenesis of several diseases in humans and animals [1,2]. Total antioxidant capacity (TAC) and antioxidant enzymes, glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD), are commonly used markers of antioxidant status and oxidative stress [1,3,4]. Tomsič et al: Plasma total antioxidant capacity and activities of blood glutathione peroxidase and superoxide dismutase determined in healthy dogs by using commercially available kits the overall antioxidant status of plasma and body fluids. Measuring TAC may help in the assessment of the physiological, environmental, and nutritional factors of the redox status [3]. Data on the reference ranges of TAC [7], GSH-Px, and SOD [8] in healthy dogs are lacking, and there are no published accepted standard reference methods for their determination. Various measurement methods have been developed for the determination of TAC, GSH-Px and SOD; these methods differ greatly among studies, which led to discrepancies in the results [5,9,10,11,12,13,14,15,16,17,18]
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