Abstract
Previous studies have indicated that the main molecular characteristic of aging is the progressive accumulation of oxidative damages in cellular macromolecules. Proteins are one of the main molecular targets of age-related oxidative stress, which have been observed during aging process in cellular systems. Reactive oxygen species (ROS) can lead to oxidation of amino acid side chains, formation of protein-protein cross-linkages, and oxidation of the peptide backbones. In the present study, we report the age-dependent oxidative alterations in biomarkers of plasma protein oxidation: protein carbonyls (PCO), advanced oxidation protein products (AOPPs) and plasma total thiol groups (T-SH) in the Indian population and also correlate these parameters with total plasma antioxidant potential. We show an age dependent decrease in T-SH levels and increase in PCO and AOPPs level. The alterations in the levels of these parameters correlated significantly with the total antioxidant capacity of the plasma. The levels of oxidized proteins in plasma provide an excellent biomarker of oxidative stress due to the relative long half-life of such oxidized proteins.
Highlights
Oxidative damage due to impaired redox homeostasis is involved in aging and age-related diseases [1,2]
Proteins are especially vulnerable to oxidative stress; the attack of Reactive oxygen species (ROS) against proteins modifies amino acids: lysine, arginine, proline, and histidine residues generating carbonyl moieties, which has been identified as an early marker for oxidative protein damage and is used as a measure of oxidative protein dam
The use of protein carbonyls (PCO) as index of oxidative stress has some advantages in comparison with the measurement of other oxidation products because of the early formation and the relative stability of carbonylated proteins [8]
Summary
Oxidative damage due to impaired redox homeostasis is involved in aging and age-related diseases [1,2]. A certain amount of oxidative stress takes place even under normal cellular conditions; the rate of this damage increases during the aging as the efficiency of redox regulation decreases [3,4]. ROS may damage all types of biological molecules including proteins and lipids in plasma of aged subjects [5,6]. Proteins are especially vulnerable to oxidative stress; the attack of ROS against proteins modifies amino acids: lysine, arginine, proline, and histidine residues generating carbonyl moieties, which has been identified as an early marker for oxidative protein damage and is used as a measure of oxidative protein dam-. Plasma total thiol groups (T-SH) is a good reflection of excess free radical generation, since the conformation of albumin is altered, allowing -SH groups to be oxidized [10]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
