Abstract
To screen differentially expressed proteins in the blood dairy cows with inactive ovaries caused by a negative energy balance and to determine the roles of the identified proteins in the development of inactive ovaries.Holstein cows at 14 to 21 days postpartum in an intensive dairy farm were examined for their energy balance (EB) status by blood β-hydroxybutyrate (BHBA) and assigned to the inactive ovary (IO) group (n = 50) and the normal oestrus control (CON) group (n = 50) at 60 to 90 days postpartum by means of the oestrus manifestation, rectal examination and B-ultrasound examination. Fourteen differentially expressed proteins from 61 proteins in the plasma of dairy cows with IOs were identified by iTRAQ/LC-MS/MS and GO, KEGG, and PATHWAY analysis. Eleven expressed proteins were upregulated, and 3 expressed proteins were downregulated. Among the 10 differentially expressed proteins verified by Western blot or ELISA, the relative expression levels of ALDOB, IGFBP2, ITIH3 and LDHB in mixed samples and single samples were consistent with the proteomic protein results. PKM2, GPX3, ALDOB, RBP4 and AHSG were significantly different between the two groups (P < 0.05); APOA4 and SPAM1 were not significantly different (P > 0.05) but were still downregulated in the ovarian resting group. This study confirmed that 14 plasma differential proteins in the inactive ovaries of postpartum dairy cows were associated with follicular development, and these findings provide a foundation for further research on the mechanism and prevention of inactive ovaries in dairy cows.
Highlights
Inactive ovaries in the postpartum period constitutes an abnormal condition in the oestrous cycle of dairy cows, resulting in no follicles on the surfaces of ovaries or no follicular deviation[1]
In this study, iTRAQ/LC-MS/MS was used to explore differential proteins the blood of dairy cows with inactive ovaries during early lactation to determine the roles of these proteins in the development of inactive ovaries, providing a foundation for the establishment of an early warning system regarding inactive ovaries
According to the background information of the CON group and inactive ovary (IO) group in Table 1, there was no significant difference in days of milking, age, number of artificial inseminations, body condition score, or milk yield between the two groups of cows
Summary
Inactive ovaries in the postpartum period constitutes an abnormal condition in the oestrous cycle of dairy cows, resulting in no follicles on the surfaces of ovaries or no follicular deviation[1]. Inactive ovaries during early lactation in dairy cows are usually classified as Type I anoestrus postpartum based on follicular dynamics, in which the follicles may appear on the surface of the ovary but follicular growth stops[1]. This type of anoestrus increases the number of postpartum days and prolongs the calving interval of dairy cows when the anestrus time is long, from 60 to 90 days postpartum, which may bring about large economic losses to dairy farms. In this study, iTRAQ/LC-MS/MS was used to explore differential proteins the blood of dairy cows with inactive ovaries during early lactation to determine the roles of these proteins in the development of inactive ovaries, providing a foundation for the establishment of an early warning system regarding inactive ovaries
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