Plasma protein binding of phenol in dogs and rats as determined by equilibrium dialysis and ultrafiltration

Abstract

Plasma-protein binding of phenol was examined in rats with and without ether anesthesia. Ether anesthesia decreased the percentage of phenol bound by rat plasma. The phenol binding capacities of dog and rat plasma were compared using equilibrium dialysis and ultrafiltration. For the wide range of phenol concentrations studied, dog plasma had a consistently higher binding capacity than rat plasma as demonstrated by both techniques. Higher binding was obtained by ultrafiltration than by equilibrium dialysis in both dog and rat plasma. Dog and rat serum albumins were responsible for 72–73% of the phenol binding in plasma, while bovine globulins either did not bind phenol or had only minimal binding capacities.