Abstract
Tumor-associated microRNAs have been detected in serum or plasma, but whether plasma microRNA-21 (miR-21) could be a potential circulating biomarker for gastric cancer (GC) prognosis in Chinese is still uncertain. Real-time quantitative reverse transcription PCR (qRT-PCR) was employed in this study to compare the relative expression of miR-21 between pre-operative and post-operative paired plasmas from 42 patients with primary GCs. The results showed that the expression levels of miR-21 in the post-operative plasmas were significantly reduced by an average of 18.2 times in all patients when compared to the pre-operative plasmas, and by 22.1 times in the subgroup of patients without family history, while only 1.76 times in the subgroup of patients with a family history. With respect of clinicopathological characteristics, the plasma miR-21 expression was highly associated with differentiation degree and lymph node metastasis rate. The results suggested plasma miR-21 could be a novel potential biomarker for GC prognosis and evaluation of surgery outcomes, especially in patients without a family history.
Highlights
Gastroscopic screening for gastric cancer (GC) is currently the most reliable screening tool, but it is unsuitable as a first-line examination due to the invasive nature
The results showed that the expression levels of miR-21 in the post-operative plasmas were significantly reduced by an average of 18.2 times in all patients when compared to the pre-operative plasmas, and by 22.1 times in the subgroup of patients without family history, while only 1.76 times in the subgroup of patients with a family history
It was found that the expression levels of miR-21 (27.93±3.21) in the post-operative plasmas from 42 cases were significantly reduced by 18.20 times when compared to the pre-operative paired plasmas (Table 1)
Summary
Gastroscopic screening for gastric cancer (GC) is currently the most reliable screening tool, but it is unsuitable as a first-line examination due to the invasive nature. A recently discovered class of small noncoding RNAs, microRNAs (miRNAs) that regulate gene expression and serve multiple biological functions, has opened a new approach of tumor biomarkers for early cancer diagnosis (He et al, 2005). The stability of tumor-associated miRNA in blood allows it to be a novel noninvasive tumor biomarker for cancer detection. Circulating miRNAs have been detected in the plasma from patients suffering from breast, colon and gastric cancers (Huang et al, 2010; Chan et al, 2013). In this study we collected plasmas from patients with primary gastric cancer, and used quantitative reverse transcription PCR (qRT-PCR) to compare circulating miR-21 expression in pre- and post-operative paired plasmas, and studied its clinical significance in gastric carcinogenesis
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