Plasma miR-208b and miR-499: Potential Biomarkers for Severity of Coronary Artery Disease.

Wei Wang,Ying Sun,Yang Li,Lei Gao,Heng-Chen Yao,Tai Li

doi:10.1155/2019/9842427

Abstract

Aims MicroRNAs (miRNAs) are associated with the pathogenesis of coronary artery disease (CAD). The objective of this study is to explore plasma levels of miR-208b and miR-499 in CAD and analyze its association with the severity of CAD. Materials and Methods 195 consecutive CAD patients who underwent coronary angiography were enrolled in this study. Severity of coronary lesions was evaluated by the synergy between percutaneous coronary intervention with taxus and cardiac surgery score (SYNTAX) score (SS). Plasma levels of miR-208b and miR-499 were assessed by quantitative real-time polymerase chain reaction (qRT-PCR). The relationship between miR-208b and miR-499 and SS was analyzed. Results The qRT-PCR results showed that plasma levels of miR-208b and miR-499 in SS > 32 (high SS) group was higher than those in low (SS ≤ 22) and intermediate (22 < SS ≤ 32) groups. Meanwhile, plasma miR-208b and miR-499 levels were significantly positive correlated with the SS (Spearman's r = 0.535 and r = 0.407, respectively; both p < 0.001). Multivariate logistic analysis results showed that miR-208b (odds ratio [OR]: 2.069; 95% confidence interval [CI]: 1.351-3.167; p = 0.001) and miR-499 (OR: 1.652; 95% CI: 1.222-2.233; p = 0.001) were independent predictors of high SS. In receiver operating characteristic curve, the area under the curve of miR-208b and miR-499 in prediction of high SS was 0.775 and 0.713, respectively. Conclusions Higher plasma levels of miR-208b and miR-499 were positively associated with the severity of CAD, and plasma miR-208b and miR-499 can act as potential biomarkers for estimating the severity of CAD.

Highlights

Coronary artery disease (CAD) and its clinical complications is still a major cause of death in the world [1, 2]
Laboratory parameters showed the levels of creatinine (Cr), creatine kinase isoenzymes (CK-MB), and high sensitivity C-reactive protein were higher in higher SS group than in lower SS group, as well as the mortality rate during hospitalization
Plasma Levels of miR-208b and miR-499 Were Positively Correlated with the Severity of CAD

Summary

Introduction

Coronary artery disease (CAD) and its clinical complications is still a major cause of death in the world [1, 2]. Various plasma biomarkers have been proved to associate with CAD, but very few of them can provide valid information about the severity of CAD [6,7,8]. Plasma miRNAs could be considered as promising biomarkers for the diagnosis and prognosis of these diseases, especially for CAD [12, 13]. Disease Markers miR-499 increased PDCD4 expression and protected endothelial cells (ECs) from inflammatory damage during CAD by the nuclear factor-kappa B (NF-κB)/tumor necrosis factor-alpha (TNF-α) signaling pathway [18]. The role of miR-208b and miR-499 in the blood vessels has been reported, the association between levels of circulating miR-208b and miR-499 and severity of CAD has not been studied yet. We aimed to evaluate the relationship of plasma miR-208b and miR-499 levels with CAD severity

Methods

Results

Discussion