Plasma Metabolome Profiling by High-Performance Chemical Isotope-Labelling LC-MS after Acute and Medium-Term Intervention with Golden Berry Fruit (Physalis peruviana L.), Confirming Its Impact on Insulin-Associated Signaling Pathways.

Patrick Poucheret,Juan Camilo Henao-Rojas,Alberto Ángel-Martín,Vanesa Corrales-Agudelo,Fabrice Vaillant,Natalia Moreno-Castellanos,Katalina Muñoz-Durango

doi:10.3390/nu13093125

Abstract

Purpose: Golden berry (Physalis peruviana L.) is an exotic fruit exported from Colombia to different countries around the world. A review of the literature tends to demonstrate a hypoglycaemic effect with an improvement in insulin sensitivity after oral ingestion of fruit extracts in animal models. However, little is known about their potential effects in humans, and very little is known about the mechanisms involved. This study aimed at identifying discriminant metabolites after acute and chronic intake of golden berry. Method: An untargeted metabolomics strategy using high-performance chemical isotope-labelling LC-MS was applied. The blood samples of eighteen healthy adults were analysed at baseline, at 6 h after the intake of 250 g of golden berry (acute intervention), and after 19 days of daily consumption of 150 g (medium-term intervention). Results: Forty-nine and 36 discriminant metabolites were identified with high confidence, respectively, after the acute and medium-term interventions. Taking into account up- and downregulated metabolites, three biological networks mainly involving insulin, epidermal growth factor receptor (EGFR), and the phosphatidylinositol 3-kinase pathway (PI3K/Akt/mTOR) were identified. Conclusions: The biological intracellular networks identified are highly interconnected with the insulin signalling pathway, showing that berry intake may be associated with insulin signalling, which could reduce some risk factors related to metabolic syndrome. Primary registry of WHO.

Highlights

Introduction distributed under the terms andGolden berry (Physalis peruviana L.) is a fruit of high commercial importance in someAfrican and Latin American countries, where it is locally consumed and often exported to northern markets, mainly Europe and the US [1]
The present study is the first investigation reported in the international literature regarding the effects of golden berries (Physalis peruviana L.) on insulin-pathway and anti-diabetic potential in a human model
The intake of golden berry fruit seems to be associated with insulin-related biological networks, as well as the epidermal growth factor receptor (EGFR)

Summary

Introduction

Introduction distributed under the terms andGolden berry (Physalis peruviana L.) is a fruit of high commercial importance in someAfrican and Latin American countries, where it is locally consumed and often exported to northern markets, mainly Europe and the US [1]. Folk medicine attributes antispasmodic, diuretic, antiseptic, sedative, and analgesic effects to the fruit [2]. The fourth study was conducted on 26 young human adults [8], and the authors reported that golden berry intake induced a postprandial decrease in glycaemia following a per os glucose challenge. Less documented is an additional potential positive impact of golden berry fruit consumption on oxidative stress and inflammatory processes and status. Most of these studies were conducted in vitro or using animal models, and data on human cohorts are very scarce. Very little is known about the mechanisms and compounds involved in the observed effects

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