Plasma membrane and cell wall properties of an aspen hybrid (Populus tremula × tremuloides) parenchyma cells under the influence of salt stress

Aleksandra Muszyńska,Ewa U Kurczynska,Katarzyna Jarocka

doi:10.1007/s11738-014-1490-3

Abstract

The effect of salinity on cell turgor, plasma membrane permeability and cell wall elasticity has been measured in petioles of an aspen hybrid using the cell pressure probe. Control plants were grown in soil without the addition of NaCl and treated plants were grown in soil with 50 mM of NaCl for 1, 2, 3 and 4 weeks. In parenchyma cells from Populus tremula × tremuloides petioles with an increased level of NaCl in the soil: (a) turgor pressure was reduced after 1 week of treatment but afterward it was similar to untreated plants, (b) the value of elastic modulus of the cell walls increased, and (c) hydraulic conductivity of the plasma membrane of treated plants decreased in comparison to untreated ones. No histological differences and distribution of JIM5 antibody between the petioles of plants grown under salinity and the untreated were found. In cell walls of parenchyma and collenchyma from plants grown under salinity, the presence of pectic epitopes recognized by JIM7 antibodies was increased in comparison to the control plants. The obtained results indicate that under salt stress the permeability of water through plasma membrane is disturbed, cell walls became more rigid but the turgor pressure did not change.

Highlights

Salt stress is one of the major abiotic stress factors that affect growth of plants worldwide
In parenchyma cells from Populus tremula 9 tremuloides petioles with an increased level of NaCl in the soil: (a) turgor pressure was reduced after 1 week of treatment but afterward it was similar to untreated plants, (b) the value of elastic modulus of the cell walls increased, and (c) hydraulic conductivity of the plasma membrane of treated plants decreased in comparison to untreated ones
The objective of the present study was to evaluate the changes in cell turgor, hydraulic conductivity of plasma membrane and elastic modulus of the cell walls of parenchyma cells from leaf petiole of aspen hybrid in control plants and plants watered with 50 mM solution of sodium chloride, to answer the question if there is any relationship between salt stress and physical properties of plasma membrane and cell wall

Summary

Introduction

Salt stress is one of the major abiotic stress factors that affect growth of plants worldwide. Too high concentrations of chloride and sodium ions in soil cause reduction of water available for plants in the first phase of salt stress (Yeo et al 1991). This stage is caused by the increased osmotic forces that retain water in the soil solution and cause reduction of water potential in the soil. Related to these changes, reduced water absorption by plants causes reduction in turgor pressure and inhibition of cell elongation (Hock and Elstner 2004). Sodium chloride causes ionic stress that is related to ion balance in every single cell in the plant body (Hasegawa et al 2000)

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