Abstract
This experiment aims to evaluate the correlation between lipid peroxidation levels in serum and seminal plasma in equines. Also, it investigates the lipid peroxidation in extended semen samples and its effects and sperm motility during a 72 hr refrigeration period. Blood and semen were collected from fertile Crioulo stallions. Serum and seminal plasma lipid peroxidation levels were analyzed by thiobarbituric acid reactive substances (TBARS) immediately after semen collection. After addition of extender (hour = 0), diluted semen was refrigerated and stored at 5 °C. Semen analyses, TBARS and catalase activity were performed in extended semen at 0, 24, 48, and 72 hours. We noted that levels of plasma lipid peroxidation can be used as an indicative of seminal oxidative stress. Also, lipid peroxidation does not increase substantially during semen storage. Lipid peroxidation and the antioxidant enzyme catalase do not seem to be the major cause of loss and motility and consequently reduction in fertility in stallion semen during storage for 72 h at 5 °C.
Highlights
Semen characteristics and quality can vary greatly among stallions within the breeding population
Damage to the spermatozoa plasma membrane results in the irreversible loss of motility and/or fertilizing capability (Baumber et al, 2000; Morris et al, 2000; Ortega Ferrusola et al, 2009; Dos Santos Hamilton et al, 2016). Since these cells have low levels of reactive oxygen species (ROS) scavengers, seminal plasma protects ejaculated equine sperm from the adverse effects of ROS, and the removal of seminal plasma during processing may increase the susceptibility of sperm to oxidative stress (Ball et al, 2001)
There was no correlation between lipid peroxidation and progressive motility on extended semen (p > 0.05)
Summary
Semen characteristics and quality can vary greatly among stallions within the breeding population. Damage to the spermatozoa plasma membrane results in the irreversible loss of motility and/or fertilizing capability (Baumber et al, 2000; Morris et al, 2000; Ortega Ferrusola et al, 2009; Dos Santos Hamilton et al, 2016). Since these cells have low levels of reactive oxygen species (ROS) scavengers, seminal plasma protects ejaculated equine sperm from the adverse effects of ROS, and the removal of seminal plasma during processing may increase the susceptibility of sperm to oxidative stress (Ball et al, 2001)
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