Plasma levels of galectin-3 in residents of the Podillya region of Ukraine without signs of cardiovascular pathology carriers of different variants of the coding gene (LGALS-3, rs 2274273)

Abstract

Annotation. The purpose of the study is to investigate the plasma level of galectin-3 in postmenopausal women without signs of cardiovascular pathology and its possible association with the structural and functional parameters of the myocardium in carriers of different polymorphic variants of the coding gene (LGALS-3, rs2274273). 67 women of postmenopausal age (average age 56.43±0.64 years) without signs of cardiovascular pathology residents of the Podillya region of Ukraine were examined. In addition to the general clinical examination, the research used the enzyme immunoassay method to determine the level of galectin-3 in blood plasma, genotyping of the LGALS3 gene at the rs2274273 locus by means of polymerase chain reaction, and ultrasound of the heart. Statistical processing of the obtained results was carried out using the package of statistical programs SPSS, STATISTICA v. 10.0. By means of correlation analysis with the calculation of Spearman's rank correlation coefficients (r), an assessment of the possible association of the plasma level of the biomarker and the structural and functional indicators of the heart was carried out. The critical value of the significance level (p) was ≤0.05. In the examined population of women, there was no significant difference between the frequency of occurrence of the GA and GG genotypes carriers of the galectin-3 gene locus rs2274273: 49.25% (n=33) and 40.30% (n=27), respectively (p>0.05). The AA genotype variant was found in 10.45% (n=7) of individuals, which is significantly less frequent than both the GA and GG genotypes (p<0.01). The frequency of distribution of genotypes corresponded to the Hardy-Weinberg equilibrium. The average level of galletin-3 in blood plasma in women was 6.68±0.30 ng/ml, it was within the generally accepted norms and did not reliably differ from the corresponding indicators in men of the same region. No significant difference in plasma concentrations of galectin-3 was found in carriers of different polymorphic variants of the galectin-3 gene. Correlation between the level of galectin-3 in the blood plasma and most indicators of intracardiac and systemic hemodynamics in women without cardiovascular pathology was not found. In the future, it is planned to study the formation of prerequisites for chronic heart failure (CHF) in women with EH based on indicators of the plasma level of galectin-3 in carriers of polymorphic variants of the coding gene (LGALS-3, rs2274273).