Abstract
A sensitive and simple method for assay of 17α-hydroxyprogesterone based on competitive protein binding technique has been developed. The steroid was isolated by liquid-gel chromatography on a hydrophobic Sephadex derivative. A mixture of n-hexane/2-methylpropan-2-oI 9:1 was used as eluent in 300 × 5 sized columns. The minimum detection limit was 0.1 ng per ml plasma. Only 0.5 ml of plasma was required for the analysis. Progesterone was assayed by competitive protein binding subsequent to purification in the same system as used for 17α-hydroxyprogesterone. 17β-Oestradiol was measured using a radioimmunoassay (Johansson, this congress).
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