Abstract
BackgroundAs a member of peroxiredoxin (PRX) family, PRX3 is predominantly located in mitochondria and plays an important role of free radical scavenging. Since a body of evidence demonstrated the involvement of PRX3 in insulin secretion, insulin sensitivity, and glucose metabolism, the present study was conducted to investigate the role of PRX3 in the pathogenesis of polycystic ovarian syndrome (PCOS) featured in insulin resistance.MethodsEnzyme-linked immunosorbent assay was performed to detect plasma PRX3 in PCOS patients and control subjects. Levels of reactive oxygen species (ROS) and oxidized PRXs were detected in mouse islet cells treated with gradient glucose.ResultsWe did not find significant difference of fasting plasma PRX3 between PCOS patients and controls. No association was noticed between fasting plasma PRX3 and fasting plasma glucose or insulin. After oral glucose tolerance test (OGTT), PCOS patients showed higher levels of both glucose and insulin as compared to controls. The plasma level of PRX3 was significantly increased at 2 h and began to fall back at 3 h of OGTT. There was a one-hour time lag of peak values between plasma PRX3 and insulin, and the plasma PRX3 at 2 h was positively correlated with the insulin level at 1 h of OGTT of PCOS patients. In addition, the level of ROS was significantly elevated at 1 h and oxidized PRX3 was increased dramatically at 2 h of 16.7mM glucose stimulation in mouse islet cells.ConclusionIt seems that PRX3 does not show its antioxidant function under baseline conditions. Instead, PRX3 responds to oxidative stress induced by rapid increase of insulin and glucose in patients with PCOS.
Highlights
As a member of peroxiredoxin (PRX) family, PRX3 is predominantly located in mitochondria and plays an important role of free radical scavenging
The obesity rate, the levels of body mass index (BMI), fasting insulin (FINS), and HOMA-insulin resistance (IR) were significantly higher in polycystic ovarian syndrome (PCOS) patients than in controls
To further understand the difference of plasma PRX3 between PCOS patients and controls, we sub-grouped the subjects according to the IR and BMI respectively
Summary
As a member of peroxiredoxin (PRX) family, PRX3 is predominantly located in mitochondria and plays an important role of free radical scavenging. Since a body of evidence demonstrated the involvement of PRX3 in insulin secretion, insulin sensitivity, and glucose metabolism, the present study was conducted to investigate the role of PRX3 in the pathogenesis of polycystic ovarian syndrome (PCOS) featured in insulin resistance. Polycystic ovarian syndrome (PCOS) is one of the most common female endocrine disorders affecting about 5– 10% women of reproductive age [1]. Insulin resistance (IR), hyperinsulinaemia, hyperglycaemia, glucose intolerance, dyslipidaemia, and obesity are frequently found in PCOS patients [2, 3]. Afterwards, considerable investigations demonstrated the role of PRX3 in glucose tolerance, insulin secretion, and IR. PRX3 plays a key role in maintaining mitochondrial homeostasis and increasing insulin sensitivity. PRX3-deficiency in mice exhibited metabolic disorders including impaired glucose tolerance, increased IR, and obesity. Since a large proportion of patients with PCOS present obesity and IR, the present study is conducted to link plasma PRX3 with IR of PCOS patients
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