Plasma ketosteroid studies in normal mice.

Abstract

ZIMMERMANN (1), Dumazert and Valensi (2), and Gardner (3) described methods for the measurement of neutral 17-ketosteroids 17-Ks) in human plasma. Migeon and Plager (4) described a method for the fractionation and measurement of 17-Ks in human plasma This report concerns the measurement of 17-Ks in mouse plasma. The procedure was developed to facilitate the study of the relationship between plasma 17-Ks and tumor growth in transplantable mouse tumors. The method to be described has been applied to normal male and female Swiss Webster mice, adrenalectomized male and female Swiss Webster mice, ACTH stimulated Swiss Webster mice, normal female DBA2 mice, and normal female C-57 black mice. materials 1. 2N. alcoholic KOH prepared as described by Wilson (5). 2. Meta-dinitrobenzene recrystallized from alcohol. A 0.2% solution is prepared in redistilled ethanol. 3. Dehydroepiandrosterone standards, 100, 200, 400 μg./ml. ethanol. 4. Purified corticotrophin gel, 40 U.S.P. units/ml. The National Drug Company, Philad...