PLASMA GLUTATHIONE S-TRANSFERASE π1-1 AND α1-1 LEVELS IN PATIENTS WITH BLADDER CANCER

Abstract

No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Dec 2000PLASMA GLUTATHIONE S-TRANSFERASE π1-1 AND α1-1 LEVELS IN PATIENTS WITH BLADDER CANCER CHRIS L. BERENDSEN, THEO P.J. MULDER, and WILBERT H.M. PETERS CHRIS L. BERENDSENCHRIS L. BERENDSEN More articles by this author , THEO P.J. MULDERTHEO P.J. MULDER More articles by this author , and WILBERT H.M. PETERSWILBERT H.M. PETERS More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)66982-0AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Transitional cell carcinomas of the human bladder and many gastrointestinal tumors often contain high amounts of the detoxification enzyme glutathione S-transferase π (GSTP1-1). Elevated levels of GSTP1-1 have been found in serum and plasma from patients with gastrointestinal, lung or head and neck cancer. GSTP1-1 and glutathione S-transferase α (GSTA1-1) have been reported to be increased in 10 of 15 patients (67%) with bladder cancer. We evaluate the role of GSTP1-1 and GSTA1-1 as plasma tumor markers in 50 patients with bladder cancer before and after treatment. Materials and Methods: Blood from patients with bladder cancer was sampled in ethylenediaminetetraacetic acid tubes. Plasma GSTA1-1 and GSTP1-1 were measured using the sensitive and specific sandwich enzyme-linked immunosorbent assay. Results: Respective plasma GSTA1-1 and GSTP1-1 levels were above the upper normal reference limit in 2 (4%) and 14 (28%) of the 50 patients with bladder cancer. No significant decrease in plasma GSTA1-1 or GSTP1-1 was noted in matched pairs of plasma samples collected before and after treatment. Conclusions: In contrast to earlier reports, only a limited number of patients with bladder cancer had increased plasma GSTA1-1 or GSTP1-1, which did not decrease after tumor resection. These findings argue against the use of GSTP1-1 or GSTA1-1 as plasma markers for bladder cancer. References 1 : The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit Rev Biochem Mol Biol1995; 30: 445. Google Scholar 2 : Nomenclature for human glutathione transferases. Biochem J1992; 282: 305. Google Scholar 3 : Preparation, characterization and properties of glutathione S-transferases. In: . Edited by . New York: Wiley J and Sons1985: 297. Google Scholar 4 : The isoenzymes of glutathione S-transferase. In: Advances in Enzymology. Edited by . New York: Wiley J and Sons1985: 357. Google Scholar 5 : Glutathione S-transferases: biomedical applications. Adv Clin Chem1993; 30: 282. Google Scholar 6 : Glutathione transferases and cancer. Crit Rev Biochem Mol Biol1992; 27: 337. Google Scholar 7 : Glutathione S-transferase activity and subunit composition in transitional cell cancer and mucosa of the human bladder. Urology1997; 49: 644. Google Scholar 8 : Enhanced glutathione S-transferase activity and glutathione content in human bladder cancer. Follow up study: influence of smoking. J Urol1993; 149: 1452. Google Scholar 9 : Glutathione S-transferase activity in human superficial transitional cell carcinoma of the bladder. Cancer1990; 9: 2064. Google Scholar 10 : Enzyme immunoassay for human serum glutathione S-transferase pi using monoclonal and polyclonal antibodies. Cancer J1989; 2: 225. Google Scholar 11 : Serum glutathione S-transferase-pi as a tumor marker for gastrointestinal malignancies. Cancer1989; 63: 317. Google Scholar 12 : Elevation of the placental glutathione S-transferase form (GST-pi) in tumor tissues and the level in sera of patients with cancer. Cancer Res1989; 49: 5225. Google Scholar 13 : Serum glutathione S-transferase-pi level as a tumor marker for non-small cell lung cancer. Cancer1994; 73: 1377. Google Scholar 14 : Radioimmunoassay for plasma glutathione S-transferase-pi and its clinical application in gastrointestinal cancer. Cancer1995; 76: 1363. Google Scholar 15 : Significance of glutathione S-transferase-pi as a tumor marker in patients with oral cancer. Cancer1992; 70: 2381. Google Scholar 16 : Isoenzyme-specific quantitative immunoassays for cytosolic glutathione transferases and measurement of the enzymes in blood plasma from cancer patients and in tumor cell lines. Biochim Biophys Acta1994; 1225: 223. Google Scholar 17 : Sandwich ELISA for glutathione S-transferase Alpha 1-1: plasma concentrations in controls and in patients with gastrointestinal disorders. Clin Chem1996; 42: 416. Google Scholar 18 : Measurement of glutathione S-transferase P1–1 in plasma; pitfalls and significance for screening and follow-up of patients with gastrointestinal cancer. Cancer1997; 80: 873. Google Scholar 19 : Hepatocellular integrity in liver donors and recipients indicated by glutathione transferase alpha. Transplantation1996; 61: 904. Google Scholar 20 : Glutathione S-transferase alpha as a marker for hepatocellular damage in pre-eclampsia and HELLP syndrome. Lancet1995; 345: 1571. Google Scholar From the Department of Urology, Atrium Medical Center Heerlen, Heerlen and Department of Gastroenterology, University Hospital St. Radboud, Nijmegen, The Netherlands© 2000 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 164Issue 6December 2000Page: 2126-2128 Advertisement Copyright & Permissions© 2000 by American Urological Association, Inc.Keywordsglutathione transferasetumor markers, biologicalenzyme-linked immunosorbent assaybladder neoplasmsMetricsAuthor Information CHRIS L. BERENDSEN More articles by this author THEO P.J. MULDER More articles by this author WILBERT H.M. PETERS More articles by this author Expand All Advertisement PDF downloadLoading ...