Abstract
Iron disorder and abnormal expression of hepcidin play important roles in many diseases, but it is still unclear in type 2 diabetes mellitus (T2DM). We aimed to assess iron, ferritin and hepcidin levels in plasma of with or without T2DM and evaluated increased body iron stores as risk factor for developing T2DM. Plasma samples were collected from 88 participants, who were categorized into 2 groups based on the presence or absence of T2DM. Demographics and general health parameters were recorded. Chemiluminescence microparticle immunoassay and enzyme-linked immunosorbent assay were used to detect iron, ferritin and hepcidin concentrations. The geometric mean±SD of the plasma level of hepcidin, ferritin, iron and insulin among T2DM comparing with that of healthy controls were evaluated. Plasma ferritin and hepcidin levels in T2DM group were higher than in the control group (P < 0.05). The geometric mean ± SD of hepcidin and ferritin for T2DM were 41.1±23.3 μg/L and 227.2±156.1 ŋg/L respectively; higher than the 15.2±2.3 μg/L and 114.4±60.4 ŋg/L of controls respectively. There was a significant associated between high level of plasma hepcidin (OR=2.75) and ferritin (OR=2.24); with T2 DM. In conclusion: the regulation of body iron, an essential but also toxic element is strictly controlled by a small peptide hormone hepcidin. The present data demonstrated that the higher hepcidin level in diabetic patients may be due to that higher ferritin, the elevated hepcidin might have adaptive value through down-regulated iron absorb and play an important role in pathogenesis of T2 DM.
Highlights
Type 2 diabetes mellitus (T2DM) is characterized by chronic hyperglycemia caused by impaired insulin secretion and/or insulin resistance[1]
In view of the fact that ferritin is generally used as a marker of body iron stores, this study suggested that increased body iron stores is a risk factor for T2DM3
In current study there was a highly significant association between high level of serum hepcidin concentration (>75 μg/dl) with T2DM by means of associated odds ratio equal to 2.75 times comparing with healthy controls, with χ2 equal to 26.7, and p value less than 0.001 (Table 3)
Summary
Type 2 diabetes mellitus (T2DM) is characterized by chronic hyperglycemia caused by impaired insulin secretion and/or insulin resistance[1]. Laboratory Tests and degradation of the iron exporter ferroportin in these cells[9], which indicates that the iron overload is Ferritin and hepcidin were tested in serum samples using chemiluminescence microparticle immunoassay further aggravated by inadequate hepcidin in T2DM. The serum hepcidin/ferritin ratio chemiluminescent reaction was measured as relative light units (RLUs) Data analysis as it represents both body iron load and adequacy of hepcidin production for a given iron load[14]. The analysis of data was done by Epi Info version 6 statistical program (CDC, Atlanta, USA), where the to assess iron, ferritin and hepcidin levels in serum of chi-square (χ2) and probability value (p) was calculated with or without T2DM and evaluated increased body for the test of significance by comparing the geometric iron stores as risk factor for developing T2DM Since mean±SD of the plasma level of hepcidin, ferritin, iron ferritin is commonly used as a marker of body iron and insulin among cases (type 2 DM) comparing with stores. A p value < 0.05 was considered we recruited 46 T2DM patients, and 42 healthy significant
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
