Plasma cxc-chemokine ligand 10, 12 and 16 levels correlate significantly with patients' clinical characteristics in idiopathic pulmonary arterial hypertension

Abstract

Plasma CXC-Chemokine Ligand 10, 12 and 16 Levels correlate significantly with patients' clinical characteristics in Idiopathic Pulmonary Arterial Hypertension. Purpose: To compare the plasma levels of CXC-Chemokine Ligand 10 (CXCL10), CXC-Chemokine Ligand 12 (CXCL12), CXC-Chemokine Ligand 16 (CXCL16) and Growth Differentiation Factor 15 (GDF-15) in Idiopathic Pulmonary Arterial Hypertension (IPAH) patients and healthy controls, and to investigate the correlation between these biomarkers and clinical characteristics in IPAH patients. Methods: Patients with IPAH conformed by right heart catheterization and age/sex matched volunteers were enrolled prospectively in this study. Venous blood samples were collected immediately after right heart catheterization. Data of World Health Organization Function Class, six minites walk distance (6MWD), echocardiography and hemodynamic parameters were collected. Plasma levels of the interest biomarkers were measured by enzyme-linked immunosorbent assay (ELISA). Results: We recruited 61 IPAH patients and 20 controls, plasma CXCL10, CXCL12, CXCL16 and GDF-15 concentrations were increased significantly in patients compared with controls (202.79±104.58 pg/ml vs 103.56±65.00 pg/ml, 2656.55±459.52 pg/ml vs 2323.02±400.87 pg/ml, 2.83±0.62 ng/ml vs 2.28±0.47 ng/ml, and 810.80±466.20 pg/ml vs 331.69±109.04 pg/ml). Levels of CXCL10 correlated significantly with Right End-Diastolic Ventricular Diameter (RVEDD), mean right atrial pressure (mRAP), right ventricular cardiac index (RVCI) and NT-proBNP. Concentration of CXCL12 correlated significantly with 6MWD, mRAP, RVCI and NT-proBNP, in addition, CXCL16 correlated significantly with 6MWD and N-Terminal Pro Brain Natriuretic Peptide (NT-proBNP). The ROC curves analyses demostrated that CXCL10 was the best for best distinguishing CI <2.0L min-1 m21 among the parameters, and GDF-15 had no significance in identifing CI <2.0L min-1 m-2. Conclusions: Plasma levels of CXCL10, CXCL12 and CXCL16 are increased in IPAH patients. Elavation of plasma CXCL10 and CXCL12 can indicate right ventricular dysfunction in patients with IPAH.