Plasma Antioxidant Enzyme Levels in Acute Coronary Syndrome

Abstract

had increased by more than 100% and neuronal excitation was observed within the paraventricular nucleus of the hypothalamus (PVN), as determined by Fra-protein expression. In contrast, similar levels of nNOS positive cellswere observed in PVNof the shamandMI rats. These experiments suggest that sympathetic activation occurs early in the progression of heart failure, independent of changes in nNOS regulation in the PVN. http://dx.doi.org/10.1016/j.hlc.2012.08.028 Distribution of G s and Co-localisation with Calcium Handling Proteins in Rat Cardiomyocytes M.L. Munro, D. Baddelely, C. Soeller Department of Physiology, School ofMedical Sciences, University of Auckland Background: The β-adrenergic pathway modulates cardiac function via phosphorylation of proteins involved in excitation-contraction-coupling, including RyR and SERCA.G s is one of the components of this pathway and it has been suggested that proteins in this pathway form signalosomes, which may localise to caveolae. Methods: Isolated rat cardiomyocytes were immunolabelled for G s with caveolin-3, RyR or SERCA and imaged using either confocal laser scanning microscopy or superresolution localisation microscopy. taneous coronary intervention. Impedance aggregometry in whole blood was used to measure platelet reactivity in response to both arachidonic acid and ADP stimulation. Plasma GPx and SOD activity levels were measured using commercially available colourimetric assay kits. In 76 ACS patients we report that there was a significant correlation betweenplatelet reactivity andGPx activity levels (p< 0.05) in response to arachidonic acid stimulation. Further investigation into themechanismof this relationship is the focus of this ongoing study. http://dx.doi.org/10.1016/j.hlc.2012.08.030 Super-resolution Imaging of Cardiac Sarcoplasmic ReticulumATPase inRelation toMyofibrilDistribution inRat