Abstract

FOLLOWING prolonged adaptation to cell cultures, several isolates of African swine fever virus (ASFV) produce cytopathic effects in pig kidney (PK) cells1–3. We have found that at least two such strains, Uganda3 and Hinde1, consistently produce plaques in monolayers of PK cells under solid overlays; this observation has led to the development of a quantitative technique using any of three PK cell strains (PK/A, PK/C and PK/13) developed by us during a period of 3 years.

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