Abstract
Metabolomics is now considered a wide-ranging, sensitive and practical approach to acquire useful information on the composition of a metabolite pool present in any organism, including plants. Investigating metabolomic regulation in plants is essential to understand their adaptation, acclimation and defense responses to environmental stresses through the production of numerous metabolites. Moreover, metabolomics can be easily applied for the phenotyping of plants; and thus, it has great potential to be used in genome editing programs to develop superior next-generation crops. This review describes the recent analytical tools and techniques available to study plants metabolome, along with their significance of sample preparation using targeted and non-targeted methods. Advanced analytical tools, like gas chromatography-mass spectrometry (GC-MS), liquid chromatography mass-spectroscopy (LC-MS), capillary electrophoresis-mass spectrometry (CE-MS), fourier transform ion cyclotron resonance-mass spectrometry (FTICR-MS) matrix-assisted laser desorption/ionization (MALDI), ion mobility spectrometry (IMS) and nuclear magnetic resonance (NMR) have speed up precise metabolic profiling in plants. Further, we provide a complete overview of bioinformatics tools and plant metabolome database that can be utilized to advance our knowledge to plant biology.
Highlights
It has wide applications ranging from investigating the stress-specific metabolites for different climatic stresses, evaluating candidate metabolic gene functions to analyzing the biological mechanism in plant cells, and dissecting the genotype-phenotype relationship in response to the various biotic and abiotic stresses
This review provides an overview of different sample collection, harvesting methods, storage, and sample preparation in the plant metabolomics experiments
The complete elucidation of physio-biochemical and molecular mechanisms underlying plant developmental and stress-responsive biology primarily depends on the comprehensive investigations using omics techniques that make metabolomics more applicable in agriculture sciences
Summary
Metabolomics is one of the fastest developing and attractive disciplines of the omics field, with huge potential and prospects in crop improvement programs. Several integrated technologies and methodologies such as mass spectrometry (MS) based methods, including gas chromatography-mass spectrometry (GC-MS), liquid chromatography mass-spectroscopy (LC-MS), capillary electrophoresismass spectrometry (CE-MS), fourier transform ion cyclotron resonance-mass spectrometry (FTICR-MS) matrix-assisted laser desorption/ionization (MALDI), ion mobility spectrometry (IMS) and nuclear magnetic resonance (NMR) are used for large-scale analysis of highly complex mixtures of plant extracts [6] These analytical methods have shown their potential in many plant species, including halophytes, medicinal plants, and food crops such as Salicornia brachiata, Cuminum cyminum, Plantago ovata, Solanum lycopersicum, Oryza sativa, Triticum aestivum, and Zea mays [7,8,9,10,11,12,13,14] (Table 1). This review describes the plant metabolome (primary and secondary metabolites), metabolomics in genetically modified (GM) crops, including different analytical techniques, bioinformatics tools, and plant metabolome database
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
