Abstract
Synthesis of nanomaterials may involve various routes including physical, chemical and biological approaches. Here, the biological green route was chosen to prepare silver nanoparticles from silver salts to avoid the requirement of costly instruments and involvement of hazardous chemicals as well. To make the process clean and green, leaf extract of parsley (Petroselinum crispum) was used to synthesize Ag nanoparticles at room temperature. The formation of Ag-nanoparticles was monitored by UV–Vis spectroscopy. The presence of silver in the sample and its crystalline nature were verified by X-ray diffraction (XRD) analysis. The size distribution profile and particle size in the suspension were manipulated from dynamic light scattering (DLS) pattern. The shape, size and morphology of the biogenic nanoparticles were studied using high resolution transmission electron microscope (TEM). Fourier transform infra-red spectroscopy was used to detect the biomolecules responsible for reduction of silver ions. These biogenic Ag-nanoparticles showed appreciable antibacterial efficacy against three bacteria—Klebsiella pneumoniae, Escherichia coli and Staphylococcus aureus.
Highlights
Research on advanced nanomaterials of noble metals like silver has gained a lot of interest among scientists during the past decades due to their unique properties that originate from high number of surface atoms and their versatile utilities and innovative applications in various engineering fields like catalysis (Santos et al 2012), optical (Das et al 2010) and antimicrobial activity (Soo-Hwan et al 2011; Venkatesham et al 2014; El-Nour et al 2010), etc
Biological methods using biological microorganism, plant extract, and enzymes have proved itself an easy, cost-effective and ecofriendly alternative synthesis route of metallic nanoparticle compared to conventional procedures (Li et al 2007; Mittal et al 2013; Tamulya et al 2013)
The Ag nanoparticle formation by reducing Ag? ions during exposure of parsley (Petroselinum crispum) leaf extract was investigated by UV–Vis spectroscopy
Summary
Research on advanced nanomaterials of noble metals like silver has gained a lot of interest among scientists during the past decades due to their unique properties that originate from high number of surface atoms and their versatile utilities and innovative applications in various engineering fields like catalysis (Santos et al 2012), optical (Das et al 2010) and antimicrobial activity (Soo-Hwan et al 2011; Venkatesham et al 2014; El-Nour et al 2010), etc. Synthesis method of nanoparticles plays an important role in its technological advancement as it is the primary step to tune their physical, electronic, and optical properties of the synthesized nanoparticles by varying size, shape and surface chemistry (Wiley et al 2007; Sharma et al 2009; Naik et al 2002). Antibacterial efficacy of these biosynthesized Agnanoparticles was tested against three bacteria—Klebsiella pneumoniae, Escherichia coli and Staphylococcus aureus
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