Abstract
Plantlets were obtained by organogenesis from cultured anthers of Populusdeltoides (Bartr.). Anthers formed callus in the dark on modified Murashige and Skoog medium supplemented with 9.0 μM 2,4-dichlorophenoxyacetic acid and 4.7 μM kinetin. Anther calli were differentiated into shoots by sequential transfer in the light onto Murashige and Skoog medium containing 4.4 μM benzylamino purine and 1.1 μM naphthaleneacetic acid for 4 weeks, followed by several transfers to woody plant medium with 2.2 μM benzylamino purine and 1.1 μM naphthaleneacetic acid. The shoots that formed were rooted by excising and transferring to woody plant medium supplemented with 1.0 μM indole-3-butyric acid. A few of these plants were found to be haploid. Two plants developed male terminal inflorescences, but died shortly thereafter.
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